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Cy5 NHS Ester PEG Cas 146368-15-2 For Targeted Drug Delivery

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China Wuhan Borenpharm Co., Ltd. certification
China Wuhan Borenpharm Co., Ltd. certification
We tested the 2,4,5-trimethyl-2,5-dihydro-1,3-thiazole in our assay and the results look good. It produced the expected stress response in our mice.

—— Kevin

Cy5 NHS Ester PEG Cas 146368-15-2 For Targeted Drug Delivery

Cy5 NHS Ester PEG Cas 146368-15-2 For Targeted Drug Delivery
Cy5 NHS Ester PEG Cas 146368-15-2 For Targeted Drug Delivery

Large Image :  Cy5 NHS Ester PEG Cas 146368-15-2 For Targeted Drug Delivery Get Best Price

Product Details:

Place of Origin: China
Brand Name: Borenpharm
Certification: www.borenpharm.com
Model Number: BK02138

Payment & Shipping Terms:

Minimum Order Quantity: 1g
Price: negotiable
Packaging Details: 1g 5g 10g 25g 50g
Delivery Time: Within 24 hours
Supply Ability: in stock
Detailed Product Description
CAS#: 146368-15-2 M.F.: C32H39ClN2O2
M.W.: 519.12 Appearance: Solid
Purity: 95% Min Storage: -20℃ For Long Term, Keep Cool And Dry
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Cy5 NHS Ester PEG

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NHS Ester PEG 146368-15-2

Cy5 With Cas.146368-15-2​​​ Of NHS ester PEG Is Applied In Bioconjugation

Item#:
BK02138
CAS#:
146368-15-2
M.F.:
C32H39ClN2O2
M.W.:
519.12
Appearance:
Solid
Purity:
95%
Storage:
-20℃ for Long Term, Keep cool and dry

 

Cy5 is a small organic molecule, and is typically conjugated to proteins via primary amines (i.e., lysines). Usually, between 3 and 7 Cy5 molecules are conjugated to each antibody (optimal is usually 5); higher conjugations can result in solubility problems as well as internal quenching (and reduced brightness). Thus, an antibody will usually be conjugated in several parallel reactions to different amounts of Cy5, and the resulting reagents will be compared for brightness (and background stickiness) to choose the optimal conjugation ratio. Cy5 is typically excited by the 633 nm line of HeNe laser, and emission is collected at 680 nm.

 

Cy5 is covalently coupled to primary amines (lysines) of the immunoglobulin.

Dissolve the Cy5 in anhydrous DMSO immediately before use, at a concentration of 10 mg/ml. Do not delay between weighing out the Cy5 and dissolving it in DMSO; likewise, do not delay the addition of the solubilized material to the antibody.

For the optimal ratio of 5:1, add 40 µg Cy5 per mg of antibody; mix immediately. (See notes above about using different molar rations of Cy5 to antibody).

Wrap the tube in foil; incubate and rotate at room temperature for 1 hour.

Remove the unreacted Cy5 and exchange the antibody into "Storage Buffer" by gel filtration or dialysis.

Contact Details
Wuhan Borenpharm Co., Ltd.

Contact Person: cathy

Tel: 13240061617

Fax: 86-27-59218822

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